Composite

Part:BBa_K2443011:Design

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-18)


LeuRS optimized for expression in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 343
    Illegal NgoMIV site found at 1534
    Illegal AgeI site found at 1004
    Illegal AgeI site found at 1031
    Illegal AgeI site found at 1085
    Illegal AgeI site found at 1162
    Illegal AgeI site found at 1934
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for use in Escherichia coli. All regulatory elements are also optimal for use within Escherichia coli



Source

see basic parts

References